Native rhizobia efficient in nitrogen fixation in Leucaena leucocephala in Rio Grande do Sul, Brazil
Abstract
The selection of promising native rhizobia, nitrogen (N)-fixing is important for pasture-leucaena integrated production for livestock production. In Rio Grande do Sul (RS), Brazil, there is little information on native isolates of rhizobia with symbiotic capacity with leucaena plants. In this sense, the objective of this research was to select native isolates of rhizobia, efficient in the biological N fixation in leucaena plants in RS state. Soil and nodule samples were taken from different locations in RS state, and then in the laboratory leucena seeds were used as a trap to capture rhizobia. An experiment under greenhouse conditions was conducted to evaluate the efficiency of the rhizobia authenticated as symbionts of leucaena. The shoot dry mass, dry mass of the root, number of nodules and the N accumulated of the part of the plant (Nac) were determined. The results showed that the selected rhizobia isolates Leu01 and Leu02 authenticated as symbionts of leucaena provided increased to the shot mass and N accumulation in leucena plants, similar to the treatment non-inoculated that received nitrogen fertilization. This isolated could be promising in future studies in greenhouse and field of this legume.
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