IN VITRO PROPAGATION OF Acacia mangium Willd
Abstract
Acacia (Acacia mangium Willd) is one of the most planted forest species because of the quality of its wood; however, and despite its importance is not easy to find juvenile plant material in adult trees to be used as a source for vegetative propagation. The present research had as objective to develop a micropropagation protocol for acacia from preexisting meristems. The explants consisted of stem section isolated from three months old plants maintained in under greenhouse condition sand shoots from in vitrogerminatedseeds. Surface disinfection was done with different sodium hypochlorite (NaOCl) concentrations plus antibiotics. Disinfected shoots were established in semisolid MS with different BAP concentrations (0; 0,44; 0,88 and 2,22 µM). Multiplied shoots were rooted using different NAA and IBA amounts and finally shoots with, or without, roots were transferred to ex vitro conditions to evaluate field adaptation. The data showed that 1,0% NaOCl and cefalexina (2 mg L-1) allowed the highest percentage of non-contaminated explants (67%). Explants cultured with 2,22 µM BAP produced the highest number of new shoots, while 2,69 µM ANA induced more roots per shoot. An 87% survival was observed.
Downloads
Languages:
es;enReferences
GALIANA, A., BALLE, P., GUESSAN KANGA, A. and DOMENACH, A. Nitrogen fixation estimated by the N natural abundance method in Acacia mangium Willd Inoculated with Bradyrhizobium sp. and grown in silvicultural conditions. Soil Biology & Biochemistry, 34(2), 2002, p. 251-262.
TONINI, H. e VIEIRA, B. Desrama, crescimento e predisposição à podridão-do-lenho em Acacia mangium. Pesquisa Agropecuária Brasileira, 41(7), 2006, p. 1077-1082.
PREECE, D. y BROOK, R. Acacia mangium: Un árbol importante para llanuras tropicales. Hoja Informativa. Una publicación de la Red de Información sobre Árboles para Bosques, Fincas y Comunidades (FACT Net) [online]. Available: www.winrock.org/forestry/factnet.htma (citado febrero 12 de 2012).
OBREGÓN, C. La Acacia mangium una especie promisoria. Revista M&M [online]. Available: http://www.revistam.com/rev49/especies.pdf2007 (citado 14 marzo 2012).
"CARRIZOSA, M. y SERRANO, C. Sistemas modelo para la micropropagación y conservación de especies forestales. Memorias de IV Congreso """"La investigación en la Universidad Javeriana"""". Bogotá (Colombia): Pontificia Universidad Javeriana, 1996, p. 261-272."
MURASHIGE, T. and SKOOG, F. A revised medium for rapid growth and bioassays with tobaco tissue cultures. Plant Physiology, 15, 1962, p. 473-497.
GIRIJASHANKAR, V. Micropropagation of multipurpose medicinal tree Acacia auriculiformis. Journal of Medicinal Plants Research, 5(3), 2011, p. 462- 466.
ABBAS, H., MUHAMMAD, Q. and BEENA, N. Rapid in vitro multiplication of Acacia nilotica subsp. hemispherica, a critically endangered endemic taxon. Pakistan Journal of Botany, 42(6), 2010, p. 4087-4093.
CORREIA, D. and CORTEZZI, M. In vitro propagation of blackwattle (Acacia meamsii De Wild). National Center of Forest Research, 1995, p. 117-125.
BONGA, M. and DURZAN, D. Tissue culture in forestry. Martinus Nijhoff Publishers, 1985, p. 420.
BONGA, M. and VON ADERKAS, P. In vitro culture of trees. Netherlands, Kluwer Academic Publishers Forestry Sciences, 1992, p. 38, 236.
SAITO, Y., KOJIMA, K., IDE, Y. and SASAKI, S. In vitro propagation from axillary buds of Acacia mangium, a legume tree in the tropics. Plant tissue culture letters, 10(2), 1993, p. 163-168.
DARUS, H. Micropropagation of Acacia mangium from aseptically germinated seedlings. Journal of Tropical Forest Science, 3(3), 1991, p. 204 – 208.
IMELDA, M., ERLYANDARI, F. and SUKIMAN, H. Effects of Rhizobial Inoculation on the early growth of Acacia mangium in the field. Berita Biology, 2006, p. 69-73.
NANDA, R., DAS, P. and ROUT, G. In vitro clonal propagation of Acacia mangium Willd and its evaluation of genetic stability through RAPD marker. Annals of Forest Science, 61(4), 2004, p. 381–386.
SUÁREZ, I., ESPITIA, M. y PERTUZ, I. Efecto de auxinas y citocininas en la multiplicación y enraizamiento in vitro de Stevia rebaudiana Bertoni. Fitotecnia Colombiana, 6(2), 2006, p. 1-8.
MONTEUUIS, O. and BON, M. Influence of auxins and darkness on in vitro rooting of micropropagated shoots from mature and juvenile Acacia mangium. Plant cell, tissue and organ culture, 63, 2000, p.173-177.
TODA, T., TAJIMA, M. and BRINI, P. Tissue culture of Acacia auriculiformis and Acacia hibrid. Bulletin of the National Forest Tree Breeding Center, 13, 1995. p. 81-94.
HUANG, F., KHAYRI J. and GRUR, E. Micropropagation of Acacia mearnsii In vitro. Cellular and Developmental Biology, 30, 1994, p. 70-74.
SUÁREZ, I., JARMA, A. y ÁVILA, M. Desarrollo de un protocolo para propagación in vitro de Roble (Tabebuia rosea Bertol DC). Revista Temas Agrarios, 11(2), 2006, p. 52 - 62.
AHUJA, M. Micropropagation á la carte in: Ahuja MR. Micropropagation of woody plants. Kluwer Academic Publishers, 41, 1993, p. 1-8.
Spanish
English
.png)
